• Oxidation of ammonia to nitrite by bacterial nitrite. This process is called nitritasi.
• Oxidation of compounds nitrite to nitrate by bacteria of nitrate. The process is called nitratasi.
Rhizobium of the inoculation on soybean seed yield increase of 31% (Norman, 1943), while Lynch and Sears (1952) found that 89 of 108 experimental units soybean fruits indicated that inoculation increased production output. Generally, land that does not contain rhizobia require pre-inoculation to obtain high production in the first years after planting, while in the soil that naturally contains rhizobia effective, pre-inoculation is an attempt to overcome the failure of production. Not all legumes that berbintil tie up N with the same content. White Clover and lucerna which is the most efficient pepolongan nemambat N.